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Tue, 21 Nov

|

Saint Lucia

Cryo-Correlative Light and Electron Microscopy (Cryo-CLEM) and Cryo-Electron Tomography (Cryo-ET) (2 Day)

Convenors: Dr Nicholas Ariotti/ Dr Stephen D. Carter/ Dr Matthias Floetenmeyer

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Cryo-Correlative Light and Electron Microscopy (Cryo-CLEM) and Cryo-Electron Tomography (Cryo-ET) (2 Day)
Cryo-Correlative Light and Electron Microscopy (Cryo-CLEM) and Cryo-Electron Tomography (Cryo-ET) (2 Day)

Time & Location

21 Nov 2023, 9:00 am – 22 Nov 2023, 5:00 pm

Saint Lucia, St Lucia QLD 4072, Australia

About the event

This will be an introductory 2-day hands-on workshop designed for beginners to learn the practical elements of conducting cryo-genic correlative light and electron microscopy experiments on adherent cells. The course will touch on the cell culture, cryo-preservation, cryo-transfer, cryo-fluorescence imaging, cryo-EM data acquisition and image re-registration.

Day 1

Session 1.

Introductory Theory - CryoCLEM

Speakers: Dr Nick Ariotti & Stephen D. Carter (University of Glasgow)

Practical Session 1.

Cell Culture

Session 2.

Theory Session 2 - Sample Preparation

Practical Session 2.

Sample preparation - Plunge Freezing

Sample Transfer – Clipping transfer loading

Session 3.

Practical Session 3.

Fluorescence Microscopy and CryoStage setup

Data Acquisition – cryofLM

Day 2

Session 4.

Theory Session 3 – Electron Tomography

Practical Session 4.

Cryotilt series acquisition

Session 5.

Theory Session 4 – Image re-registration

Practical Session 5.

Image correlation and re-registration image processing

Session 6.

Wrap-up

Tickets

  • Cryo-Correlative Light and EM

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