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Tue, 21 Nov

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Saint Lucia

Cryo-SEM (2 Day)

Convenor: Na’ama Koifman

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Cryo-SEM (2 Day)
Cryo-SEM (2 Day)

Time & Location

21 Nov 2023, 9:00 am – 22 Nov 2023, 5:00 pm

Saint Lucia, Building 75,Cnr College Rd &, Cooper Rd, St Lucia QLD 4072, Australia

About the event

Cryo-SEM is a useful direct imaging  technique of soft matter systems, including but not limited to complex liquid  systems, biological systems, gels and polymers. In this 2-day workshop we will  review the basic concepts of cryo-SEM and all the equipment involved in this  multi-step technique. The practical part of the workshop will include specimen  preparation demonstration by high pressure freezing, freeze fracturing, cryo  transfer and cryo imaging by a high-resolution SEM.

First day – 21 November

Morning session

Ø Introduction to cryo-SEM – background, types of samples, workflow, available systems at CMM

Ø Specimen preparation for cryo-SEM – Types of preparations, sample holders, common artefacts, and strategies to avoid them.

Ø Discussion of challenges of cryo-SEM users and future developments at various facilities.

Afternoon session

Ø Demonstration of high pressure freezing on HPM-100 or HPM-010.

Ø Tour around the facility and introduction to the different systems.

Second day – 22 November

Morning session

Ø Preparation of the SEM for a cryo-SEM session – cooling down

Ø BAF060 Freeze fracture system demonstration – Loading, transferring, fracturing, etching, coating (if needed)

Ø Transfer fractured specimens into the Sigma microscope.

Ø Imaging under cryo-conditions – low-dose imaging, identification of artefacts, choosing correct imaging conditions.

Ø Unloading samples from the microscope and end of session.

Open discussion with participants.

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  • Cryo-SEM

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